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Original Research
5.
Studies on the duration of immunity induced in
cattle after natural FMD infection and post
vaccination with bivalent oil vaccine - Ehab El-Sayed, Wael Mossad, Samir Mohamed Ali,
Mohamed Shawky
Vet World. 2012; 5(10): 603-608
doi:
10.5455/vetworld.2012.
603-608
Abstract
Aim:
To detect the proper time for vaccination of
infected calves after the onset of clinical signs
and revaccination for previously vaccinated animals
with bivalent FMD montanide ISA206 inactivated
vaccine. Materials and Methods: Twenty calves
naturally infected with foot and mouth disease (FMD)
showing clinical signs represented by lesions on the
tongue, buccal mucosa and feet. Samples were
collected from these calves including tongue
epithelial and oropharyngeal fluid (OP). The
causative virus was isolated from such samples
through inoculation of baby mice and typed by
indirect Sandwich ELISA and was confirmed in 2
tongue epithelia samples using PCR.
Results:
The serum antibody titers were determined using SNT
and ELISA in the sera of the 20 infected calves
revealing that the infected farms should be
vaccinated with inactivated bivalent FMD vaccine for
(type O and A) Adjuvanted with montanide ISA206
after 32 weeks from the appearance of clinical
signs. In addition the FMD immune status was
monitored in 2 farms in EL-Fayoum and El-Sharkia
Governorates where 50 calves in each farm were
vaccinated with the bivalent FMD inactivated vaccine
adjuvaned with montanide ISA206 and serum samples
were collected monthly from these animals to
determine their immune status using SNT and ELISA.
The obtained results showed that vaccinated calves
with the bivalent FMD montanide ISA206 inactivated
vaccine should be revaccinated after 36 weeks post
vaccination.
Conclusions: naturally infected
calves should be vaccinated on the 32 weeks post
infection while vaccinated calves should be
revaccinated on the 36 weeks post vaccination.
Keywords: bivalent montanide ISA 206 vaccine,
ELISA, Foot and Mouth disease, infection, SNT,
vaccination
